Journal: Archives of Gynecology and Obstetrics

Article Title: Glycosylated fibronectin as a first trimester marker for gestational diabetes

doi: 10.1007/s00404-020-05670-8

Figure Lengend Snippet: a Glycosylated fibronectin concentrations in BMI subgroups of 20–25 and 30–35 kg/m 2 in the study and the control group. b Effect of smoking on concentrations of glycosylated fibronectin in the control and GDM groups. c Multiple of median (MoM) values for fibronectin and glycosylated fibronectin between control and GDM groups. d Multiple of median (MoM) values for adiponectin and glycosylated adiponectin between control and GDM groups

Article Snippet: Calibrators for both assays were made from recombinant human adiponectin (1065-AF, R&D Systems, Abingdon, UK).

Techniques: Control

Journal: Pediatric diabetes

Article Title: Relationship of Adiponectin and Leptin with Autoimmunity in Children with New Onset Type 1 Diabetes (T1D): A Pilot Study

doi: 10.1111/pedi.12267

Figure Lengend Snippet: Longitudinal Adiponectin Levels

Article Snippet: Adiponectin and Leptin assays Serum total adiponectin levels were determined by radioimmunoassay (Human adiponectin RIA kit, Linco Research, St Charles, Missouri, USA).

Techniques:

Journal: Pediatric diabetes

Article Title: Relationship of Adiponectin and Leptin with Autoimmunity in Children with New Onset Type 1 Diabetes (T1D): A Pilot Study

doi: 10.1111/pedi.12267

Figure Lengend Snippet: Racial differences in longitudinal adipocytokine values

Article Snippet: Adiponectin and Leptin assays Serum total adiponectin levels were determined by radioimmunoassay (Human adiponectin RIA kit, Linco Research, St Charles, Missouri, USA).

Techniques:

Journal: EMBO Molecular Medicine

Article Title: Photoreceptor glucose metabolism determines normal retinal vascular growth

doi: 10.15252/emmm.201707966

Figure Lengend Snippet: The mouse neonates received intraperitoneal injection of STZ and schematic graph shows mouse retinal vessel development after birth (left). GCL, ganglion cell layer; INL, inner nuclear layer; ONL, outer nuclear layer. Representative images of the deep vascular network in isolectin‐stained (red, top) retinal whole mounts of HAR and controls. Scale bar, 50 μm (top) and 1 mm (bottom). The total retinal area is outlined in yellow and deep vascular coverage is highlighted in red (bottom, grayscale). Quantification of deep vascular network of HAR and controls. n = 17–27 retinas/group. Data presented as mean ± SEM, unpaired t ‐test. Blood glucose ( n = 19–27 retinas/group), serum insulin (ELISA) ( n = 4–7 pooled samples/group) and triglyceride (TG) concentrations ( n = 7/group) of HAR and controls. Data presented as mean ± SEM, unpaired t ‐test. Data information: See also Fig .

Article Snippet: All serum samples were diluted 1:306 and APN levels assayed using a human adiponectin ELISA kit (E091M, Mediagnost, Reutlingen, Germany).

Techniques: Injection, Staining, Enzyme-linked Immunosorbent Assay

Journal: EMBO Molecular Medicine

Article Title: Photoreceptor glucose metabolism determines normal retinal vascular growth

doi: 10.15252/emmm.201707966

Figure Lengend Snippet: A Left: serum APN levels (ELISA) ( n = 8–9 retinas/group); right: retinal apn (qRT–PCR) ( n = 6 retinas/group) of HAR and controls. B Left: retinal cross‐sectional layers for laser capture microdissection (LCM: DAPI for nuclei, blue; isolectin for vessels, red); center: mRNA levels of AdipoR1 and AdipoR2 in retinal neuronal layers and vessels. n = 3 pooled retinas/group. Right: AdipoR1 mRNA in HAR and control retinas. n = 5 retinas/group. C Control and HAR eyes in WT and Apn −/− mice. Left: representative images of DAPI‐stained hyaloid vessels (blue). Right: quantification of preserved hyaloid vessels branching from the hyaloid artery (white arrow). Scale bar, 1 mm. n = 8–30 retinas/group. D Left: Fundus photograph focused to show persistent hyaloid vessels (white arrows) (green, fluorescein AK‐FLUOR) in WT and Apn −/− mice at P30 ( n = 37–48 retinas/group); right: pie graph of percentage of eyes examined with persisting hyaloid. E Left: In WT and Apn −/− representative images of deep retinal vasculature (lectin, red) in whole‐mounted retinas; right: quantification of deep vessels ( n = 14–22 retinas/group). F, G Left: Representative images of deep retinal vasculature (lectin, red) in whole‐mounted retinas of WT hyperglycemic (HAR) mice with recombinant mouse APN treatment (F) ( n = 6–10 retinas/group) or with AdipoRon treatment (G). Right: quantification of deep retinal vasculature ( n = 7–14 retinas/group). Data information: Scale bars, 50 μm (B, E–G) or 1 mm (C). Data presented as mean ± SEM; unpaired t ‐test (A, B, E–G) or ANOVA (C). See also Fig .

Article Snippet: All serum samples were diluted 1:306 and APN levels assayed using a human adiponectin ELISA kit (E091M, Mediagnost, Reutlingen, Germany).

Techniques: Enzyme-linked Immunosorbent Assay, Quantitative RT-PCR, Laser Capture Microdissection, Control, Staining, Recombinant

Journal: Journal of Clinical Biochemistry and Nutrition

Article Title: Utility of hemoglobin A1c in detecting risk of type 2 diabetes: comparison of hemoglobin A1c with other biomarkers

doi: 10.3164/jcbn.19-16

Figure Lengend Snippet: Correlation between the glucose and inslurin resistance during fasting and OGTT and fasting levels of multi-biomaekers

Article Snippet: HbA1c, glucose, insulin, leptin, adiponectin, RBP4, glycoalbumin, and hs-CRP were measured using commercially available ELISA kits {HbA1c: RAPIDIA Auto HbA1c-L [Fujirebio Inc. (Tokyo, Japan)]; glucose: Cica liquid GLU J [KANTO CHEMICAL Co., Inc. (Tokyo, Japan)]; insulin: Lumipulse Presto Insulin (Fujirebio Inc.); leptin: Human leptin RIA kit [Millipore Co., Inc. (Tokyo, Japan)]; adiponectin: CircuLexTM Human adiponectin ELISA Kit CY-8050 [MBL Co., Ltd. (Nagano, Japan)]; RBP4: CircuLex Human RBP4 ELISA Kit (MBL Co., Ltd.); glycoalbumin: Lucia GA-L [Asahi KASEI Pharma Co., Inc. (Tokyo, Japan)]; and hs-CRP: CircuLexTM Human HS-CRP ELISA Kit CY-8071 (MBL Co., Ltd.)}.

Techniques:

Journal: African Health Sciences

Article Title: Role of plasma adiponectin /C-reactive protein ratio in obesity and type 2 diabetes among African Americans

doi: 10.4314/ahs.v17i1.13

Figure Lengend Snippet: Metabolic and physical characteristics by gender (Mean ± S.E.M)

Article Snippet: Fasting blood glucose concentration was measured with One Touch Ultra monitoring system, hs-CRP analyses were carried out with an Immulite 2000 analyzer (Siemens Medical Solutions Diagnostics, Erlanger, Germany), Insulin was detected using the Human Insulin ELISA kit from Millipore (St.Charles, Missouri, USA), Adiponectin was detected using the Human Adiponectin sandwich immunoassay kit from Meso Scale Discovery (Gaithersburg, MD, USA) and lipid profiles (Total Cholesterol, HDL-C, LDL-C and TG) were determined in the Clinical Laboratory at the National Institutes of Health Department of Laboratory Medicine using an LX-20 analyzer (Beckman, San Diego, CA).

Techniques:

Journal: African Health Sciences

Article Title: Role of plasma adiponectin /C-reactive protein ratio in obesity and type 2 diabetes among African Americans

doi: 10.4314/ahs.v17i1.13

Figure Lengend Snippet: Partial correlations between adiponectin, CRP, physical and metabolic variables by gender (Women: n = 77; Men: n = 34)

Article Snippet: Fasting blood glucose concentration was measured with One Touch Ultra monitoring system, hs-CRP analyses were carried out with an Immulite 2000 analyzer (Siemens Medical Solutions Diagnostics, Erlanger, Germany), Insulin was detected using the Human Insulin ELISA kit from Millipore (St.Charles, Missouri, USA), Adiponectin was detected using the Human Adiponectin sandwich immunoassay kit from Meso Scale Discovery (Gaithersburg, MD, USA) and lipid profiles (Total Cholesterol, HDL-C, LDL-C and TG) were determined in the Clinical Laboratory at the National Institutes of Health Department of Laboratory Medicine using an LX-20 analyzer (Beckman, San Diego, CA).

Techniques: Fat

Journal: African Health Sciences

Article Title: Role of plasma adiponectin /C-reactive protein ratio in obesity and type 2 diabetes among African Americans

doi: 10.4314/ahs.v17i1.13

Figure Lengend Snippet: Plasma adiponectin, CRP, and the adiponectin to CRP ratio based on normal %body fat and fasting glucose (Women = 23; Men = 16), high %body fat and normal fasting glucose (Women = 30; Men = 12) and normal/high % body fat/high fasting glucose (Women = 43; Men = 21) (Mean ± S.E.M)

Article Snippet: Fasting blood glucose concentration was measured with One Touch Ultra monitoring system, hs-CRP analyses were carried out with an Immulite 2000 analyzer (Siemens Medical Solutions Diagnostics, Erlanger, Germany), Insulin was detected using the Human Insulin ELISA kit from Millipore (St.Charles, Missouri, USA), Adiponectin was detected using the Human Adiponectin sandwich immunoassay kit from Meso Scale Discovery (Gaithersburg, MD, USA) and lipid profiles (Total Cholesterol, HDL-C, LDL-C and TG) were determined in the Clinical Laboratory at the National Institutes of Health Department of Laboratory Medicine using an LX-20 analyzer (Beckman, San Diego, CA).

Techniques: Clinical Proteomics